Journal: Pathogens and Disease

Article Title: Typing of feces-derived Candida albicans strains using a novel seven-locus microsatellite panel reveals associations with yeast phenotype in individuals with inflammatory bowel disease

doi: 10.1093/femspd/ftaf001

Figure Lengend Snippet: Enzymatic activity of feces-derived C. albicans strains reveals no differentially active enzymes. (A–E) Enzyme activity was based in triplicate for one strain per individual. Each data point represents mean activity of a strain as determined in triplicate. (A) Proteinase activity. (B) Phospholipase activity. (C) Lipase activity. (D) Esterase activity. (E) Average activity across all enzymes. Horizontal dashed line indicates activity of reference strain SC5314. (F–J) Clinical fecal and serum titers. (F) Fecal calprotectin. (G) ASCA titers, IgA immunoglobulins. (H) ASCA titers, IgG immunoglobulins. (I) Total ASCA titers. (J) Serum 1,3-β-glucan levels. (K) Correlation plot of all determined enzyme activities and clinical parameters based on Spearman r . Letters and symbols denote significance levels. Spearman r , a P < .05, b P < .005. (L) Correlation between PC2 and proteinase activity. Spearman r = 0.417, P = .043, n = 24. (M) Correlation between esterase activity and PC2. Spearman r = .408, P = .048, n = 24. Data of all included patients, except for enzyme activity ( n = 8 HV, n = 12 CD, n = 4 UC). Kruskal–Wallis or Mann–Whitney U test, * P < .05, ** P < .01.

Article Snippet: For determination of ASCAs and 1,3-β-glucans in serum, colorimetric kits were used according to the manufacturer's protocol, being the Orgentec ASCA IgA/IgG kit (Siemens Healthineers, Den Haag, The Netherlands) and FungiTell diagnostic kit (Nodia, Amstelveen, The Netherlands), respectively.

Techniques: Activity Assay, Derivative Assay, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Presence of Anti-Microbial Antibodies in Liver Cirrhosis – A Tell-Tale Sign of Compromised Immunity?

doi: 10.1371/journal.pone.0012957

Figure Lengend Snippet: Anti-microbial serological markers in patients with liver cirrhosis according to the disease severity depicted by Child-Pugh stages.

Article Snippet: Both serum IgG and IgA levels of anti- Saccharomyces cerevisiae antibodies (ASCA) were evaluated separately according to the manufacturer's protocol (ASCA IgG, ASCA IgA, QUANTA LiteTM, INOVA Diagnostics).

Techniques:

Journal: PLoS ONE

Article Title: Presence of Anti-Microbial Antibodies in Liver Cirrhosis – A Tell-Tale Sign of Compromised Immunity?

doi: 10.1371/journal.pone.0012957

Figure Lengend Snippet: A . Individual values are shown by black spots. Mean values with standard error bars are indicated in blue. Cut-off values for positivity are 25 Units for all antibodies. P <0.001 between all groups by ANOVA post hoc Scheffe for ASCA IgA and anti-OMP Plus™ IgA P = NS for ASCA IgG. B . MELD Q1-Q4 represent the groups of patients broken down by quartile: quartile1 patients have the lowest severity up to quartile4, representing patients with the highest level of severity. P <0.001 between all groups by ANOVA post hoc Scheffe for ASCA IgA and anti-OMP Plus™ IgA P = NS for ASCA IgG.

Article Snippet: Both serum IgG and IgA levels of anti- Saccharomyces cerevisiae antibodies (ASCA) were evaluated separately according to the manufacturer's protocol (ASCA IgG, ASCA IgA, QUANTA LiteTM, INOVA Diagnostics).

Techniques:

Journal: PLoS ONE

Article Title: Presence of Anti-Microbial Antibodies in Liver Cirrhosis – A Tell-Tale Sign of Compromised Immunity?

doi: 10.1371/journal.pone.0012957

Figure Lengend Snippet: Anti-microbial serological markers in cirrhotic patients according to the absence or presence of ascites.

Article Snippet: Both serum IgG and IgA levels of anti- Saccharomyces cerevisiae antibodies (ASCA) were evaluated separately according to the manufacturer's protocol (ASCA IgG, ASCA IgA, QUANTA LiteTM, INOVA Diagnostics).

Techniques:

Journal: PLoS ONE

Article Title: Presence of Anti-Microbial Antibodies in Liver Cirrhosis – A Tell-Tale Sign of Compromised Immunity?

doi: 10.1371/journal.pone.0012957

Figure Lengend Snippet: Logistic regression: Predictive factors for severe bacterial infection in patients with liver cirrhosis.

Article Snippet: Both serum IgG and IgA levels of anti- Saccharomyces cerevisiae antibodies (ASCA) were evaluated separately according to the manufacturer's protocol (ASCA IgG, ASCA IgA, QUANTA LiteTM, INOVA Diagnostics).

Techniques: Infection

Journal: PLoS ONE

Article Title: Presence of Anti-Microbial Antibodies in Liver Cirrhosis – A Tell-Tale Sign of Compromised Immunity?

doi: 10.1371/journal.pone.0012957

Figure Lengend Snippet: Summary of Cox model: factors affecting time to first severe bacterial infection.

Article Snippet: Both serum IgG and IgA levels of anti- Saccharomyces cerevisiae antibodies (ASCA) were evaluated separately according to the manufacturer's protocol (ASCA IgG, ASCA IgA, QUANTA LiteTM, INOVA Diagnostics).

Techniques: Infection